Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis
نویسندگان
چکیده
منابع مشابه
Reuse of denaturing polyacrylamide gels for short tandem repeat analysis.
Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing. To reduce the drawback of preparing gels or using expensive precast gels, we have developed a simple and rapid method to reuse gels between 2 and 8 times over a period of several days. Following the initial elec...
متن کاملShort tandem repeat analysis for human identity testing.
Short tandem repeat (STR) typing has become the workhorse of modern forensic DNA analysis. The most common form of STR typing uses laser-induced fluorescence detection of dye-labeled polymerase chain reaction (PCR) products following capillary electrophoresis (CE) size-based separation. This unit describes the techniques and marker systems most widely used around the world in constructing crimi...
متن کاملGeneral approach to analysis of polymorphic short tandem repeat loci.
Polymerase chain reaction amplification products of 22 known polymorphic short tandem repeat (STR) loci were subjected to denaturing polyacrylamide gel electrophoresis and detected using a silver staining method. Loci that amplified efficiently and revealed the fewest amplification-related artifacts with this detection method were selected for development of allelic ladders. The combination of ...
متن کاملAmplification of differentially displayed PCR products isolated from untreated denaturing polyacrylamide gels.
Analysis of differentially expressed genes has been facilitated by introduction of two related techniques: differential display (3,4) and RNA arbitrarily primed polymerase chain reaction (RAP-PCR) (10). These powerful techniques have been used to identify developmentally, environmentally and hormonally regulated genes in animals and plants (5,7–9). In each method, two or more RNAs are used as t...
متن کاملSilver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels.
Thompson, L.A. Strasheim and R.R. Burgess. 1990. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem. 265:17911-17920. 20.May, G., C. Sutton and H. Gould. 1991. Purification and characterization of Ku-2, an octamer-binding prote...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98255rr04